期刊
SEPARATION AND PURIFICATION REVIEWS
卷 52, 期 1, 页码 58-74出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15422119.2022.2038625
关键词
Supercritical fluid chromatography; amino acid; peptide; separation
This review article gives an overview of the application of supercritical fluid chromatography (SFC) techniques in the separation of amino acids and peptides, as well as modifications of mobile phases in SFC for highly polar and hydrophilic compounds. It also covers the use of SFC in chiral and achiral separations of amino acids and peptides, providing detailed explanations of possible and already performed separations for each type of compound. Different stationary phases, separation conditions, and compositions of the mobile phase are summarized.
This review article provides an overview of supercritical fluid chromatography (SFC) techniques for the separation of amino acids and peptides and modifications of mobile phases in SFC for the separation of highly polar and hydrophilic compounds. Furthermore, it covers SFC applications for the chiral and achiral separations of amino acids and peptides. In this review, detailed explanations of possible and already performed achiral and chiral separations by individual amino acids and peptides are presented. Different stationary phases, separation conditions such as backpressure and temperature, and compositions of the mobile phase with possible additives for each amino acid and peptide are summarized.
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