期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 357, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131417
关键词
Recombinant camel serum albumin; Protein-based nanocomposite; Nanozyme; Desolvation; Colorimetric detection
资金
- National Natural Science Foundation of China [U1703118]
- Natural Science Foundation of Jiangsu Province [BK20181364]
- Natural Science Foundation of Jiangsu Higher Education Institutions of China [19KJA310003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Jiangsu Shuangchuang Program
- Jiangsu Specially-Appointed Professor Project, China
Protein-based enzyme mimics have advantages of easy preparation, compatibility, and superior catalytic performance. A novel hemin-loaded nanozyme system was prepared using a stable Bactrian camel serum albumin. The obtained nanoparticles showed improved enzyme mimic activity and higher affinity to substrates, with adjustable particle size and morphology.
Protein-based enzyme mimics hold advantages of easy preparation, desirable compatibility and superior catalytic performance. Taking advantage of the remarkable stability of Bactrian camel serum albumin (CSA) compared with its counterpart in human and bovine, here a novel hemin-loaded nanozyme system was prepared through the desolvation method using a recombinant CSA (rCSA). The obtained hemin-rCSA nanoparticles exhibited improved enzyme mimic activity with higher affinity to the substrates compared with bare hemin and horseradish peroxidase. Moreover, the particle size and morphology of hemin-rCSA NPs was adjustable by the optimization of ethanol addition during the desolvation process. With the efficient encapsulation of hemin in the protein nanocomposite, a colorimetric detection of glutathione with the linear calibration concentration range of 3.2-100 mu M with an LOD of 0.667 mu M was achieved within 8 min. The available camel-derived serum albumin of recombinant form of improved stability provides a robust and manipulable protein-based nanoplatform for the molecular design of nanozyme system with enormous biomedical potentials.
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