Novel sandwich-typed electrochemical immunosensing of C-reactive protein using multiply twinned AuPtRh nanobead chains and nitrogen-rich porous carbon nanospheres decorated with Au nanoparticles

C-reactive protein (CRP) is commonly utilized as a significant biomarker for inflammation and infection, whose sensitive detection is of importance for early diagnosis of such diseases as a greatly reliable prediction. In this work, a novel sandwich-typed electrochemical immunosensor was built based on mono-dispersed nitrogen-rich porous carbon nanospheres decorated with Au nanoparticles (Au-N-CNSs) for such determination, coupled with multiply twinned AuPtRh nanobead chains (AuPtRh NBCs) as a label material. The AuPtRh NBCs were synthesized by a simple solvothermal method with micelles of a block copolymer poly(ethylene oxide)-b-poly (methyl methacrylate) (PEO(10500)-b-PMMA((18000))), which can enlarge the loading capacity, significantly catalyze the oxidation of potassium ferricyanide (K-3[Fe(CN)(6)]), and increase the conductivity, ultimately amplifying the signals. The obtained sandwich-typed immunosensor showed a broad linear range of 0.01 ng mL(-1)-100 mu g mL(-1), and a low limit of detection (LOD = 7.70 pg mL(-1)). This strategy offers certain constructive insights for both bioanalysis and early clinical diagnosis of inflammation or tissue damage.

Automated summary

In this study, a novel immunosensor was developed for the sensitive detection of CRP using Au-N-CNSs and AuPtRh NBCs. The sandwich-typed immunosensor exhibited a broad linear range and a low detection limit, providing valuable insights for early diagnosis of inflammation and tissue damage.