期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 359, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131591
关键词
Self-supported electrode; Mild-oxidation; Functionalized flexible graphite foil; Immunosensors; COVID-19
资金
- COST (European Cooperation in Science and Technology) [CA17104]
- Ca' Foscari University Venice, Italy [9L2AC3B8]
This paper presents an ultrasensitive and label-free electrochemical immunosensor for monitoring the SARS-CoV-2 spike protein. A self-supported electrode was fabricated by partially exfoliating a flexible graphitic carbon foil, which served as both an antibody immobilization matrix and electron transport channel. The immunosensor showed high reproducibility and specificity in detecting SARS-CoV-2 SP within a wide concentration range.
This paper reports on an ultrasensitive and label-free electrochemical immunosensor for monitoring the SARSCoV-2 spike protein (SARS-CoV-2 SP). A self-supported electrode, which can simultaneously serve as an antibody immobilization matrix and electron transport channel, was initially fabricated by a controlled partial exfoliation of a flexible graphitic carbon foil (GCF). Mild acidic treatment enabled the partial oxidation and exfoliation (down to a few layers) of the flexible GCF; this also provided a high percentage of oxygen functionality and an enhanced surface roughness. The substrate electrode was further functionalized with ethylenediamine (EDA) to provide a suitable platform with even a higher surface roughness, for the covalent immobilization of an anti-SARS-CoV-2 antibody. The change in the current response for the [Fe(CN)(6)](3-/4-) redox couple, induced by the binding of SARS-CoV-2 SP to the antibody immobilized on the electrode surface, was used to determine the SARS-CoV-2 SP concentration. The immunosensor thus prepared could detect SARSCoV-2 SP within 30 min with high reproducibility and specificity over a wide concentration range (0.2-100 ng/ mL). Detection limits of 25 pg/mL and 27 pg/mL were found in a phosphate buffer solution (pH 7.4), and diluted blood plasma, respectively. The immunosensor was also employed to detect SARS-CoV-2 SP in artificial human saliva.
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