期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 358, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131505
关键词
Near-infrared; Ratiometric; Alkaline phosphatase; Fluorescent probe; Imaging
资金
- National Natural Science Foundation of China [21772078, 22071200]
- Fundamental Research Funds for the Central Universities [2682020CX55, 2682021ZTPY011]
- MoE of China [XJ2021KJZK004]
- Science and Technology Department of Sichuan Province, China [2020JDRC0021]
The newly designed NIR fluorescent probe SWJT-3 is capable of ratiometric determination of alkaline phosphatase (ALP) and can be used for both in vitro and in vivo detection. It is the first NIR and ratiometric fluorescent probe for imaging ALP in mice, with a large Stokes shift and low detection limit.
A novel near-infrared (NIR) fluorescent probe (SWJT-3) was designed based on phosphate protection of the electron donating group of the hemicyanine for the ratiometric determination of ALP at 590 and 670 nm. Notably, SWJT-3 could be used in the endogenous detection of ALP in vitro and vivo. Moreover, it was the first NIR and ratiometric fluorescent probe for the imaging of ALP in mice. It has large Stokes shift (185 nm), and also showed high selectivity, low detection limit (0.87 U/L). Meanwhile, SWJT-3 exhibited obvious color change in aqueous solution which could be observed by naked-eyes. In addition, the probe displayed low lifetime (0.74 ns), high substrate affinity (K-m = 8.89 mu M) and excellent fluorescence characterization under various polarity and viscosity conditions.& nbsp;
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据