期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 820, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2022.153302
关键词
Wetland plant; Perfluorooctanoic acid (PFOA); Toxicity; FTIR; Biomolecules
资金
- National Natural Science Foundation of China [NSFC 51909107, NSFC 41877359]
- Hainan University Start-up Funding for Scientific Research (KYQD) [KYQD[ZR]-21015, KYQD[ZR]-21033]
- Guangdong Natural Science Foundation [2020A1515010448]
- Hainan Natural Science Foundation [421QN195, 421QN196]
- China Postdoctoral Science Foundation [2019M653284]
Researchers have found that exposure to perfluorooctanoic acid (PFOA) negatively affects the growth of the wetland plant Alisma orientale, leading to changes in the structures, compositions, and functions of lipids, proteins, and DNA in plant cells.
Perfluoroalkyl substances (PFASs) have been widely studied by researchers due to their environmental persistence, chemical stability and potential toxicity. Some researchers have reported the physiological and biochemical toxicity of PFASs on plants through traditional and innovative methods; however, the changes in biological macromolecules caused by PFASs are rarely studied. Here, Fourier transform infrared spectroscopy (FTIR) was used to study how exposure to perfluorooctanoic acid (PFOA) alters the structure and function of biomolecules of the wetland plant Alisma orientale. Biomass results showed that PFOA had negative effects on plant growth. FTIR results showed that PFOA could result in changes in the structures, compositions, and functions of lipids, proteins and DNA in plant cells. In the treatment groups, the ratios of CH3 to lipids and carbonyl esters to lipids increased compared with the control, while the ratios of CH2 to lipids and olefinicCH to lipids decreased, which indicated lipid peroxidation caused by PFOA exposure. Changes in the compositions and secondary structures of proteins were also found, which were indicated by the decreased ratio of amide Ito amide II and the increased ratio beta-sheet to alpha-helix in the treatment groups compared to the control. Moreover, PFOA affected the composition of DNA by promoting the B-to A-DNA transition. These results showed that the mechanism of PFOA toxicity toward plants at the biochemical level could be illustrated by FTIR.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据