Comparison of fitness effects in the earthworm Eisenia fetida after exposure to single or multiple anthropogenic pollutants

Terrestrial ecosystems are exposed to many anthropogenic pollutants. Non-target effects of pesticides and fertilizers have put agricultural intensification in the focus as a driver for biodiversity loss. However, other pollutants, such as heavy metals, particulate matter, or microplastic also enter the environment, e.g. via traffic and industrial activities in urban areas. As soil acts as a potential sink for such pollutants, soil invertebrates like earthworms may be particularly affected by them. Under natural conditions soil invertebrates will likely be exposed to combinations of pollutants simultaneously, which may result in stronger negative effects if pollutants act synergistically.Within this work we study how multiple pollutants affect the soil-dwelling, substrate feeding earthworm Eisenia fetida. We compared the effects of the single stressors, polystyrene microplastic fragments, polystyrene fibers, brake dust and carbon black, with the combined effect of these pollutants when applied as a mixture. Endpoints measured were survival, increase in body weight, reproductive fitness, and changes in three oxidative stress markers (glutathione Stransferase, catalase and malondialdehyde). We found that among single pollutant treatments, brake dust imposed the strongest negative effects on earthworms in all measured endpoints including increased mortality rates. Sublethal effects were found for all pollutants. Exposing earthworms to all four pollutants simultaneously led to effects on mortality and oxidative stress markers that were smaller than expected by the respective null models. These antagonistic effects are likely a result of the adsorption of toxic substances found in brake dust to the other pollutants. With this study we show that effects of combinations of pollutants cannot necessarily be predicted from their individual effects and that combined effects will likely depend on identity and concentration of the pollutants.

Automated summary

Terrestrial ecosystems are threatened by anthropogenic pollutants, including pesticides, fertilizers, heavy metals, particulate matter, and microplastics. This study focuses on the effects of multiple pollutants on soil-dwelling earthworms, particularly their survival, body weight, reproductive fitness, and oxidative stress. The results reveal that the combination of pollutants can have different effects than their individual effects, and the identity and concentration of the pollutants play a significant role in determining the combined effects.