Mechanism and potential risk of antibiotic resistant bacteria carrying last resort antibiotic resistance genes under electrochemical treatment

The significant rise in the number of antibiotic resistance genes (ARGs) that resulted from our abuse of antibiotics could do severe harm to public health as well as to the environment We investigated removal efficiency and removal mechanism of electrochemical (EC) treatment based on 6 different bacteria isolated from hospital wastewater carrying 3 last resort ARGs including NDM-1, mcr-1 and tetX respectively. We found that the removal efficiency of ARGs increased with the increase of both voltage and electrolysis time while the maximum removal efficiency can reach 90%. The optimal treatment voltage and treatment time were 3 V and 120 min, respectively. Temperature, pH and other factors had little influence on the EC treatment process. The mechanism of EC treatment was explored from the macroscopic and microscopic levels by scanning electron microscopy (SEM) and flow cytometry. Our results showed that EC treatment significantly changed the permeability of cell membrane and caused cells successively experience early cell apoptosis, late cell apoptosis and cell necrosis. Moreover, compared with traditional disinfection methods, EC treatment had less potential risks. The conjugative transfer frequencies of cells were significantly reduced after treatment. less than 1% of bacteria entered the viable but nonculturable (VBNC) state and less than 5% of intracellular ARGs (iARGs) turned into extracellular ARGs (eARGs). Our findings provide new insights into as well as important reference for future electrochemical treatment in removing ARB from hospital wastewater.

Automated summary

We investigated the efficiency and mechanism of electrochemical treatment in removing antibiotic resistance genes (ARGs) from bacteria isolated from hospital wastewater. We found that the removal efficiency of ARGs increased with higher voltage and longer electrolysis time, with the optimal treatment conditions being 3 V and 120 min. Scanning electron microscopy and flow cytometry revealed that electrochemical treatment altered the cell membrane permeability and induced different levels of apoptosis and necrosis. Compared with traditional disinfection methods, electrochemical treatment had lower potential risks, such as reduced bacterial conjugative transfer frequencies and minimal conversion of intracellular ARGs to extracellular ARGs.