期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 811, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2021.152341
关键词
Polyhydroxyalkanoates (PHAs); Mixed microbial consortia (MMCs); Community dynamics; Growth conditions; RDA-analysis
资金
- Bio-Economy Region Northern Netherlands cooperative (BERNN)
- European Regional Development Fund (EFRO)
- Northern Netherlands Provinces (SNN) Spatial Eco-nomic Program
- China Scholarship Council (CSC)
This study identified the relationship between growth conditions (C/N ratios and pH) and key-microbes during PHB accumulation, showing that Thauera was the most prevalent species in MMCs at pH 9. The results demonstrate the impact of C/N ratios and pH on microbial prevalence and PHB production levels, providing insights for regulating high PHB production within MMCs.
Growth conditions have been frequently studied in optimizing polyhydroxybutyrate (PHB) production, while few studies were performed to unravel the dynamic mixed microbial consortia (MMCs) in the process. In this study, the relationship between growth conditions (C/N ratios and pH) and the corresponding key-microbes were identified and monitored during PHB accumulation. The highest PHB level (70 wt% of dry cell mass) was obtained at pH 9, C/N 40, and acetic acid 10 g/L. Linking the dominant genera with the highest point of PHB accumulation, Thauera was the most prevalent species in all MMCs of pH 9, except when a C/N ratio of 1 was applied. Notably, dominant bacteria shifted at pH 7 (C/N 10) from Thauera (0 h) to Paracoccus, and subsequently to Alcaligenes following the process of PHB accumulation and consumption. Further understanding of the relationship between the structure of the microbial community and the performance will be beneficial for regulating and obtaining high PHB accumulation within an MMC. Our study illustrates the impact of C/N ratios and pH on microbial prevalence and PHB production levels using a mixed microbial starter culture. This knowledge will broaden industrial perspectives for regulating high PHB production and timely harvesting.
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