期刊
RUSSIAN CHEMICAL BULLETIN
卷 71, 期 5, 页码 1050-1056出版社
SPRINGER
DOI: 10.1007/s11172-022-3507-1
关键词
1; 3; 5-triazine; 5-phenyltetrazol-2-ylacetic acid; aziridine; DNA binding constants; antitumor activity
资金
- Ministry of Health of the Russian Federation
The interaction between the synthesized 1,3,5-triazine derivative and DNA was studied, showing efficient binding and formation of stable associates. Additionally, the tetrazole-containing derivative exhibited cytostatic effects on liver adenocarcinoma and glioblastoma cells.
({5-[4,6-Di(aziridin-1-yl)-1,3,5-triazin-2-yl]amino}-2,2-dimethyl-1,3-dioxan-5-yl)-methyl 2-(5-phenyl-2H-tetrazol-2-yl)acetate was synthesized and characterized. The interaction of the obtained tetrazole-containing 2-amino-4,6-di(aziridin-1-yl)-1,3,5-triazine derivative with DNA was studied by UV spectrophotometry, circular dichroism spectroscopy, and isothermal calorimetry. The values of binding constants (9.65 center dot 10(8) L mol(-1), UV spectrophotometry; 4.08 center dot 10(8) L mol(-1), isothermal calorimetry) of the 1,3,5-triazine derivative to DNA attests to efficient binding and formation of stable associates with the biopolymer. The addition of the tetrazole-containing 1,3,5-triazine derivative to a DNA solution has virtually no effect on the DNA melting point. The tetrazole-containing 1,3,5-triazine derivative exhibits a cytostatic effect on SK-HEP-1 human hepatic adenocarcinoma cells (half-maximal inhibitory concentration IC50 22.2 mmol L-1) and T98G human glioblastoma cells (IC50 44.9 mmol L-1).
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