期刊
RNA BIOLOGY
卷 19, 期 1, 页码 560-574出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2022.2064073
关键词
Ribosome biogenesis; 40S ribosomal subunit; ribosomal protein; rps15/uS19; yeast; chronic lymphocytic leukaemia; CLL
资金
- Austrian science fund (FWF) [P27996-B21]
- Austrian science fund [P28874-B21]
- BioTechMed-Graz Flagship project DYNIMO
- Land Steiermark
- Spanish Ministry of Science and Innovation/Agencia Estatal de Investigacion agencia estatal de investigacion AEI [PID2019-103850-GB-I00]
- Andalusian regional government [P20_00581, BIO-271]
- Spanish Ministry of Science and Innovation (BFundacion Espanola para la Ciencia y la Tecnologia) [ES-2017-080876]
- doc.fund 50 'Molecular Metabolism'
- Stadt Graz
- Austrian Science Fund (FWF) [P27996, P28874] Funding Source: Austrian Science Fund (FWF)
The small ribosomal subunit protein Rps15/uS19 is involved in both nucleolar ribosome assembly and cytoplasmic pre-40S maturation. The C-terminal tail of Rps15 plays a role in quality control during late pre-40S maturation, ensuring functional Rps15 C-terminal tail in 40S ribosomal subunits entering translation.
The small ribosomal subunit protein Rps15/uS19 is involved in early nucleolar ribosome biogenesis and subsequent nuclear export of pre-40S particles to the cytoplasm. In addition, the C-terminal tail of Rps15 was suggested to play a role in mature ribosomes, namely during translation elongation. Here, we show that Rps15 not only functions in nucleolar ribosome assembly but also in cytoplasmic pre-40S maturation, which is indicated by a strong genetic interaction between Rps15 and the 40S assembly factor Ltv1. Specifically, mutations either in the globular or C-terminal domain of Rps15 when combined with the non-essential Itv1 null allele are lethal or display a strong growth defect. However, not only rps15 Itv1 double mutants but also single rps15 C-terminal deletion mutants exhibit an accumulation of the 205 pre-rRNA in the cytoplasm, indicative of a cytoplasmic pre-40S maturation defect. Since in pre-40S particles, the C-terminal tail of Rps15 is positioned between assembly factors Rio2 and Tsr1, we further tested whether Tsr1 is genetically linked to Rps15, which indeed could be demonstrated. Thus, the integrity of the Rps15 C-terminal tail plays an important role during late pre-40S maturation, perhaps in a quality control step to ensure that only 40S ribosomal subunits with functional Rps15 C-terminal tail can efficiently enter translation. As mutations in the C-terminal tail of human RPS15 have been observed in connection with chronic lymphocytic leukaemia, it is possible that apart from defects in translation, an impaired late pre-40S maturation step in the cytoplasm could also be a reason for this disease.
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