期刊
RNA BIOLOGY
卷 19, 期 1, 页码 533-540出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2022.2062177
关键词
Promoter antisense RNA; Pol II promoter-proximal pause release; nascent RNA sequencing; CRISPR-Cas9; CRISPR-Cas13
资金
- DOD Prostate Cancer Research Program [W81XWH-16-1-0548]
This article summarizes recent technological advances in the accurate detection of PAS RNA and discusses the mechanisms of PAS RNA biogenesis, emphasizing the important role of its structure in transcription and chromatin regulation.
Omics-based technologies, which have developed rapidly over the last few decades, have generated increasing evidence demonstrating pervasive divergent transcription from RNA polymerase II (Pol II) promoters of eukaryotic genome, and indeed have raised considerable discussion as to their potential physiopathological function. Unlike many other long non-coding RNAs (lncRNAs), promoter antisense RNAs (PAS RNAs) were initially considered to be merely passive transcription by-products of active promoters. However, recent studies have begun to reveal their critical importance in a broad spectrum of biological processes. In this Review, I summarize recent technological advances that enable accurate detection of PAS RNA and discuss the mechanisms of PAS RNA biogenesis emphasizing the functional importance of its structure enabling the diverse functions of PAS RNA in transcription and chromatin regulation.
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