期刊
REPRODUCTIVE BIOMEDICINE ONLINE
卷 45, 期 1, 页码 15-18出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2022.03.026
关键词
Adenomyosis; Alpha-smooth muscle actin; Ectopic endometrium; Fibrosis; Sphingosine 1-phosphate; Sphingosine 1-phosphate receptors
资金
- Ministry of Education, Universities and Research of Italy
- Fondazione Careggi, Project on Woman's Health
This study found a significant dysregulation of the S1P signaling axis in adenomyosis, and the expression of ACTA2, which is associated with fibrosis, was positively correlated. This suggests that S1P may serve as a potential biomarker for adenomyosis.
Research question: Is sphingosine 1-phosphate (S1P) pathway involved in the process of fibrosis in adenomyosis? Design: RNA was extracted from paraffin-embedded slices collected from the ectopic endometrium of patients with nodular adenomyosis (n = 27) and eutopic endometrium of healthy controls women (n = 29). Expression of genes involved in the metabolism and signalling of S1P, and actin-alpha-2 smooth muscle, encoded by ACTA2 gene, a gene involved in fibrogenesis, was evaluated by real-time polymerase chain reaction analysis. Results: In adenomyotic samples, the expression of sphingosine kinase 1 (SPHK1), the enzyme responsible for the synthesis of S1P, and of S1P phosphatase 2 (SGPP2), the enzyme responsible for the conversion of S1P back to sphingosine, was lower (P = 0.0006; P = 0.0015), whereas that of calcium and integrin-binding protein 1, responsible for membrane translocation of SPHK1, was higher (P = 0.0001) compared with healthy controls. In S1P signalling, a higher expression of S1P receptor S1P(3) (P = 0.001), and a lower expression of S1P(2) (P = 0.0019) mRNA levels, were found compared with healthy endometrium. In adenomyotic nodules, a higher expression of ACTA2 mRNA levels were observed (P = 0.0001), which correlated with S1P(3) levels (P = 0.0138). Conclusion: Present data show a profound dysregulation of the S1P signalling axis in adenomyosis. This study also highlights that the bioactive sphingolipid might be involved in the fibrotic tract of the disease, correlated with the expression of ACTA2, suggesting its role as novel potential biomarker of adenomyosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据