期刊
PROTEOMICS
卷 22, 期 15-16, 页码 -出版社
WILEY
DOI: 10.1002/pmic.202200026
关键词
human peripheral blood mononuclear cells (PBMCs); mass spectrometry; protein biomarkers; proteomics; sample preparation
资金
- Deutsche Forschungsgemeinschaft [404521405, SFB 1389]
- Ministerstvo skolstva, vedy, vyskumu a sportu Slovenskej republiky [APVV-19-0476, ITMS2014+, 313011V446]
In this review, recent applications of proteomic analysis of PBMCs are described and promising protein biomarker candidates are emphasized.
Human peripheral blood mononuclear cells (PBMCs) represent a sentinel blood sample which reacts to different pathophysiological stimuli in the form of immunological responses/immunophenotypic changes. The study of molecular content of PBMCs can provide better understanding of immune processes giving the possibility of monitoring the health conditions of the host organism. Proteomic analysis of PBMCs can achieve mentioned goal as important immune-related biomarkers are easily accessible for analysis. PBMCs have been gaining attention in different research areas including preclinical or clinical investigations. In this review, recent applications of proteomic analysis of PBMCs are described and discussed. Approaches are divided based on different proteomic workflows such as in-gel, in-solution and on-filter modes. The effect of various diseases such as autoimmune, cancer, neurodegenerative, viral, metabolic, and various immune stimulations such as radiation, vaccine, corticosteroids over PBMCs proteome, are described with emphasis on promising protein biomarker candidates.
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