期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2118002119
关键词
YeiE; LysR-type transcriptional regulator; sulfite; hydrogen sulfide; sulfite reductase
资金
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science ICT [2019M3E5D6063871]
- NRF - Ministry of Education [2017H1A2A1042661]
- National Research Foundation of Korea [2017H1A2A1042661, 2019M3E5D6063871] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cronobacter sakazakii is an emerging pathogenic bacterium that causes various diseases in infants. The study identified YeiE as a global virulence regulator and investigated its role in the response to sulfite and its toxicity during bacterial growth. The research revealed the structural changes of YeiE in the presence of sulfite and its functional roles in H2S production and survival capability from neutrophils.
Cronobacter sakazakii is an emerging gram-negative pathogenic bacterium that causes meningitis, bacteremia, and necrotizing enterocolitis in infants and has a high mortality rate. The YeiE homolog (gpESA_01081) was identified as a global virulence regulator of bacterial pathogenesis in C. sakazakii. YeiE is a LysR-type transcriptional regulator (LTTR) composed of a DNA binding domain and a regulatory domain to recognize the unknown ligand. To reveal the molecular mechanism and function of YeiE, we determined the crystal structure of the regulatory domain of YeiE. A sulfite ion was bound at the putative ligand-binding site, and subsequent studies revealed that the sulfite is the physiological ligand for YeiE. Structural comparisons to its sulfite-free structure further showed the sulfite-dependent conformational change of YeiE. The essential role of YeiE in defending against toxicity from sulfite during the growth of C. sakazakii and Escherichia coli was examined. Furthermore, the target genes and functional roles of YeiE in H2S production and survival capability from neutrophils were investigated. Our findings provide insights into the sophisticated behaviors of pathogenic gram-negative bacteria in response to sulfite from the environment and host.
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