期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2122245119
关键词
diagnosis; prognosis; serum metabolic fingerprints; mass spectrometry; breast cancer
资金
- National Natural Science Funds [81971771, 81772802, 82073269, M-0349]
- Ministry of Science and Technology of China [2017YFE0124400, 2018YFC1312800]
- Shanghai Institutions of Higher Learning [2021-01-07-00-02-E00083]
- Shanghai Science and Technology Innovation Action Plan [20XD1402800]
- Clinical Research Plan of Shanghai Shen-kang Hospital Development Centre [SHDC2020CR2065B]
- Shanghai Rising-Star Program [19QA1404800]
- Shanghai Municipal Education Commission [ZXWF082101]
- Clinical Research Innovation Plan of Shanghai General Hospital [CTCCR-2016B05]
- Natural Science Foundation of Jiangsu Province [BK20181186]
- National Research Center for Translational Medicine Shanghai [TMSK-2021-124, NRCTM (SH) -2021-06]
- Medical-Engineering Joint Funds of Shanghai Jiao Tong University [YG2019QNA44, YG2021ZD09, YG2022QN107]
- Shanghai Science and Technology Commission [20JC1410100]
High-performance metabolic analysis using fast nanoparticle-enhanced laser desorption/ionization mass spectrometry (NPELDI-MS) was used to record serum metabolic fingerprints (SMFs) of breast cancer patients. The SMFs were used for machine learning to distinguish breast cancer from non-breast cancer, and also for constructing a metabolic prognosis scoring system. Additionally, a panel of differentially enriched metabolites and related pathways in breast cancer serum were identified.
High-performance metabolic analysis is emerging in the diagnosis and prognosis of breast cancer (BrCa). Still, advanced tools are in demand to deliver the application potentials of metabolic analysis. Here, we used fast nanoparticle-enhanced laser desorption/ionization mass spectrometry (NPELDI-MS) to record serum metabolic finger-prints (SMFs) of BrCa in seconds, achieving high reproducibility and low consumption of direct serum detection without treatment. Subsequently, machine learning of SMFs generated by NPELDI-MS functioned as an efficient readout to distinguish BrCa from non-BrCa with an area under the curve of 0.948. Furthermore, a metabolic prognosis scoring system was constructed using SMFs with effective prediction performance toward BrCa (P < 0.005). Finally, we identified a biomarker panel of seven metabolites that were differentially enriched in BrCa serum and their related pathways. Together, our findings provide an efficient serum metabolic tool to characterize BrCa and highlight certain metabolic signatures as potential diagnostic and prognostic factors of diseases including but not limited to BrCa.
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