Research Note: Effects of Bacillus coagulans X26 on the production performance, intestinal structure, short-chain fatty acids and flora composition of laying hens during the peak laying period

This study investigated the effects of Bacillus coagulans X26 on the production performance, egg quality, intestinal structure, intestinal short-chain fatty acids, and microbial diversity of laying hens during the peak laying period and explored the possibility of using B. coagulans X26 as an alternative to antibiotics. The results showed that adding 1.0 x 10(6) CFU/g B. coagulans X26 to the basal diet had the best effect: the average laying rate increased by 4.20% (P < 0.05), the survival rate increased by 7.15% (P < 0.05), the feed intake decreased by 3.06% (P < 0.05), the ratio of feed to egg decreased by 7.42% (P < 0.05), the rate of soft-broken eggshell decreased by 73.04% (P < 0.05), the average egg weight increased by 2.94% (P < 0.05), and the content of egg white protein increased by 5.77% (P < 0.05). The production performance and egg quality of laying hens reached the same level as that of hens fed a diet supplemented with chlortetracycline hydrochloride in this study, and there were significant advantages in the average laying rate and feed-egg ratio (P < 0.05). Both chlortetracycline hydrochloride and B. coagulans X26 altered the flora composition and the SCFA content of the intestinal contents; however, B. coagulans X26 also significantly increased the villus height of the ileum and the ratio of villus height to crypt depth (P < 0.05).

Automated summary

This study found that adding Bacillus coagulans X26 during the peak laying period can significantly improve the production performance and egg quality of laying hens, surpassing those fed with chlortetracycline hydrochloride. Additionally, Bacillus coagulans X26 improves intestinal structure and increases the content of short-chain fatty acids in the intestines. Therefore, Bacillus coagulans X26 has the potential to be used as an alternative feed additive to antibiotics.