Metabolic and transcriptome analysis of dark red taproot in radish (Raphanus sativus L.)

The red color in radish taproots is an important quality index and is mainly affected by anthocyanins. However, the metabolite components and gene expression underlying dark red taproot color formation in radish remain elusive. In this study, the metabolites and gene expression patterns affecting anthocyanin biosynthesis were monitored in the dark red taproots. Comparative analysis of anthocyanin metabolites between dark red taproots and white taproots indicated that pelargonin and pelargonidin 3-O-beta-D-glucoside were the most promising dark red pigments responsible for the coloration of the taproots. Transcriptomic analysis of gene expression between dark red taproots and white taproots revealed that most of genes involved in the anthocyanin biosynthesis pathway were up-regulated in dark red taproots. In particular, RsCHS and RsDFR were the two most up-regulated genes in the dark red taproots. Moreover, the higher coexpression of two R2R3-Myb transcription factors, RsMYB1 and RsMYB2, may contribute to dark red color formation. Our work documents metabolomic and transcriptomic changes related to the dark red color formation in taproots radish and provides valuable data for anthocyanin-rich radish breeding.

Automated summary

This study investigated the metabolites and gene expression patterns involved in dark red taproot color formation in radish. The findings suggest that pelargonin and pelargonidin 3-O-beta-D-glucoside are the main pigments responsible for the coloration, and most genes involved in anthocyanin biosynthesis are up-regulated in dark red taproots. The coexpression of RsMYB1 and RsMYB2 transcription factors may contribute to the formation of dark red color. This work provides valuable insights for breeding anthocyanin-rich radish.