期刊
PLOS ONE
卷 17, 期 4, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0265049
关键词
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资金
- National Key R&D Program of China [2016YFE0204400]
- Social Development Program of Jiangsu Province [BE2018626]
- Scientific Research Program of Health Committee of Wuxi City [ZM005]
Mesenchymal stromal cells (MSCs) promote wound healing and cell growth through paracrine cytokine signaling. Conditioned medium of MSCs pretreated with IFN-gamma and TNF-alpha (IT MSC-CM) showed better effects on promoting cell migration, proliferation, and activation in human dermal fibroblasts (HDFs) compared to normal MSC-CM.
Human dermal fibroblasts (HDFs) play important roles in all stages of wound healing. However, in nonhealing wounds, fibroblasts are prone to aging, resulting in insufficient migration, proliferation and secretion functions. Recent studies have suggested that mesenchymal stromal cells (MSCs) are conducive to wound healing and cell growth through paracrine cytokine signaling. In our studies, we found that conditioned medium of MSCs pretreated with IFN-gamma and TNF-alpha (IT MSC-CM) has abundant growth factors associated with wound repair. Our in vitro results showed that the effects of IT MSC-CM on promoting cell migration, proliferation and activation in HDFs were better than those of conditioned medium from mesenchymal stromal cells (MSC-CM). Moreover, we embedded a scaffold material containing IT MSC-CM and reconfirmed that cell migration and activation were superior to that in the presence of MSC-CM in vivo. Generally, PDGF-BB is perceived as a promoter of the migration and proliferation of HDFs. Moreover, a high level of PDGF-BB in IT MSC-CM was detected, according to which we guess that the effect on HDFs may be mediated by the upregulation of PDGF-BB. These studies all showed the potential of IT MSC-CM to promote rapid and effective wound healing.
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