4.7 Article

Cytological and physiological tolerance of transgenic tobacco to Cd stress is enhanced by the ectopic expression of SmZIP8

期刊

PLANT SCIENCE
卷 319, 期 -, 页码 -

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ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2022.111252

关键词

Cd; Phytoremediation; SmZIP8; Tolerance; Transgenic tobacco

资金

  1. National Natural Science Foundation of China [31901184]
  2. Natural Science Foundation of Tianjin, China [17JCYBJC22500]
  3. Doctor Foundation of Tianjin Normal University [52XB1914]

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This study found that high expression of SmZIP8 in Salix matsudana can enhance the tolerance of tobacco to Cd stress, reducing cell damage, raising mitotic indices, and reducing chromosome aberration rates. Meanwhile, transgenic tobacco also showed increased photosynthetic capacity and activated antioxidant enzymes, indicating enhanced physiological tolerance to Cd.
Zrt and Irt-like proteins (ZIPs) are responsible for transporting various divalent metal cations. However, information about the characteristics of the cellular and physiological tolerance of plant ZIPs to Cd stress is still limited. The expression levels of SmZIP8 in Salix matsudana Koidz were upregulated by Cd stress. The complete length of SmZIP8 from S. matsudana was cloned, and transgenic tobacco was obtained by Agrobacterium-mediated transformation. Then, the tolerance to Cd stress of wild-type (WT) and transgenic tobacco seedlings was analyzed and compared by studying the cytotoxicity of the root tip cells, photosynthetic parameters, histochemical staining of O-2-and H2O2, the activities of antioxidant enzymes, and malondialdehyde content under Cd stress. In comparison with WT tobacco, the ectopic expression of SmZIP8 in tobacco promoted the cytological tolerance of the transgenic tobacco to Cd stress by reducing cell damage, raising the mitotic indexes, and reducing the rate of chromosome aberration of the root cells. Meanwhile, the results of increased photosynthetic capacity, decreased oxidative damage, and activated antioxidant enzymes showed that the physiological tolerance of transgenic tobacco to Cd was enhanced. The principal component analysis for the above physiological parameters explained 96.08% of the total variance (PC1, 77.77%; PC2, 18.31%), indicating a significant difference in Cd tolerance abilities between the tobacco expressing SmZIP8 and WT tobacco. Therefore, SmZIP8 may be considered as an important genetic resource for the phytoremediation of Cd or other heavy metal pollution via the use of trans genic plants obtained through genetic transformation.

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