4.8 Article

Shedding light on silica biomineralization by comparative analysis of the silica-associated proteomes from three diatom species

期刊

PLANT JOURNAL
卷 110, 期 6, 页码 1700-1716

出版社

WILEY
DOI: 10.1111/tpj.15765

关键词

biosilica; silica morphogenesis; intrinsically disordered proteins; silaffins; GFP-tagging; frustule; fultoportula; Thalassiosira pseudonana; Thalassiosira oceanica and Cyclotella cryptica

资金

  1. Deutsche Forschungsgemeinschaft (DFG) [KR 1852/8-2, SH94/4-2]
  2. Alexander von Humboldt Stiftung
  3. European Regional Development Fund (ERDF/EFRE) [100232736]
  4. German Federal Ministry of Education and Research (BMBF) [03Z22EB1]
  5. German Research Foundation (DFG) [INST 269/731-1 FUGG]
  6. Projekt DEAL

向作者/读者索取更多资源

This study extracted silica proteins from three diatom species and found that there were no common proteins among them, and most proteins showed low similarity in sequence alignments. Bioinformatic analysis grouped these proteins into different classes based on unconventional sequence motifs, but their functions are still unclear. In vivo localization results of selected proteins suggest that proteins lacking sequence homology but sharing unconventional sequence motifs may have similar functions in diatom silica cell wall morphogenesis.
Morphogenesis of the intricate patterns of diatom silica cell walls is a protein-guided process, yet to date only very few such silica biomineralization proteins have been identified. Therefore, it is currently unknown whether all diatoms share conserved proteins of a basal silica forming machinery, and whether unique proteins are responsible for the morphogenesis of species-specific silica patterns. To answer these questions, we extracted proteins from the silica of three diatom species (Thalassiosira pseudonana, Thalassiosira oceanica, and Cyclotella cryptica) by complete demineralization of the cell walls. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis of the extracts identified 92 proteins that we name 'soluble silicome proteins' (SSPs). Surprisingly, no SSPs are common to all three species, and most SSPs showed very low similarity to one another in sequence alignments. In-depth bioinformatics analyses revealed that SSPs could be grouped into distinct classes based on short unconventional sequence motifs whose functions are yet unknown. The results from the in vivo localization of selected SSPs indicates that proteins, which lack sequence homology but share unconventional sequence motifs may exert similar functions in the morphogenesis of the diatom silica cell wall.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据