期刊
PLANT CELL
卷 34, 期 5, 页码 2038-2055出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plcell/koac064
关键词
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资金
- National Key Research and Development Program of China [2018YFD1000800]
- National Natural Science Foundation of China [32102459, 31870261, 32170246]
- Startup Fund for Young Faculty at SJTU [21X010500765]
- Zhiyuan Scholar Program at SJTU
- Natural Science Foundation of Shanghai [22ZR1431300]
- Medicine and Engineering Interdisciplinary Research Fund of SJTU [YG2021ZD07]
The tomato transcription factor module BBX20/21-HY5 activates HY5 transcription, and the HY5 protein inhibits its own gene transcription, forming an autoregulatory negative feedback loop in UV-B signaling.
In tomato (Solanum lycopersicum) and other plants, the photoreceptor UV-RESISTANCE LOCUS 8 regulates plant UV-B photomorphogenesis by modulating the transcription of many genes, the majority of which depends on the transcription factor ELONGATED HYPOCOTYL 5 (HY5). HY5 transcription is induced and then rapidly attenuated by UV-B. However, neither the transcription factors that activate HY5 transcription nor the mechanism for its attenuation during UV-B signaling is known. Here, we report that the tomato B-BOX (BBX) transcription factors SlBBX20 and SlBBX21 interact with SlHY5 and bind to the SlHY5 promoter to activate its transcription. UV-B-induced SlHY5 expression and SlHY5-controlled UV-B responses are normal in slbbx20 and slbbx21 single mutants, but strongly compromised in the slbbx20 slbbx21 double mutant. Surprisingly, UV-B responses are also compromised in lines overexpressing SlBBX20 or SlBBX21. Both SlHY5 and SlBBX20 bind to G-box1 in the SlHY5 promoter. SlHY5 outcompetes SlBBX20 for binding to the SlHY5 promoter in vitro, and inhibits the association of SlBBX20 with the SlHY5 promoter in vivo. Overexpressing 35S:SlHY5-FLAG in the WT background inhibits UV-B-induced endogenous SlHY5 expression. Together, our results reveal the critical role of the SlBBX20/21-SlHY5 module in activating the expression of SlHY5, the gene product of which inhibits its own gene transcription under UV-B, forming an autoregulatory negative feedback loop that balances SlHY5 transcription in plants. Tomato transcription factor module BBX20/21-HY5 activates HY5 transcription, and HY5 protein inhibits its own gene transcription, forming an autoregulatory negative feedback loop in UV-B signaling.
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