期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 20, 期 7, 页码 1257-1273出版社
WILEY
DOI: 10.1111/pbi.13802
关键词
seabuckthorn assembly; paleopolyploidizations; karyotype reconstruction; fatty acid; ascorbic acid
资金
- Special Fund for Forest Scientific Research in the Public Welfare [201504103]
- National Natural Science Foundation of China [U2003116]
- Fundamental Research Funds of CAF [CAFYBB2020SZ001-2]
This study presents the first chromosome-level genome assembly of seabuckthorn, providing insights into its evolutionary trajectory and identifying key genes controlling the content of ascorbic acid and fatty acids in the fruit. These findings offer novel clues for understanding phytochemical innovation in seabuckthorn and provide valuable resources for exploring the evolution of the Elaeagnaceae family and molecular breeding.
Plants of the Elaeagnaceae family are widely used to treat various health disorders owing to their natural phytochemicals. Seabuckthorn (Hippophae rhamnoides L.) is an economically and ecologically important species within the family with richness of biologically and pharmacologically active substances. Here, we present a chromosome-level genome assembly of seabuckthorn (http://hipp.shengxin.ren/), the first genome sequence of Elaeagnaceae, which has a total length of 849.04 Mb with scaffold N50 of 69.52 Mb and 30 864 annotated genes. Two sequential tetraploidizations with one occurring similar to 36-41 million years ago (Mya) and the last similar to 24-27 Mya were inferred, resulting in expansion of genes related to ascorbate and aldarate metabolism, lipid biosynthesis, and fatty acid elongation. Comparative genomic analysis reconstructed the evolutionary trajectories of the seabuckthorn genome with the predicted ancestral genome of 14 proto-chromosomes. Comparative transcriptomic and metabonomic analyses identified some key genes contributing to high content of polyunsaturated fatty acids and ascorbic acid (AsA). Additionally, we generated and analysed 55 whole-genome sequences of diverse accessions, and identified 9.80 million genetic variants in the seabuckthorn germplasms. Intriguingly, genes in selective sweep regions identified through population genomic analysis appeared to contribute to the richness of AsA and fatty acid in seabuckthorn fruits, among which GalLDH, GMPase and ACC, TER were the potentially major-effect causative genes controlling AsA and fatty acid content of the fruit, respectively. Our research offers novel insights into the molecular basis underlying phytochemical innovation of seabuckthorn, and provides valuable resources for exploring the evolution of the Elaeagnaceae family and molecular breeding.
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