期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 20, 期 9, 页码 1716-1729出版社
WILEY
DOI: 10.1111/pbi.13842
关键词
Eragrostis tef; lodging; SEMIDWARF-1; CRISPR; Cas9; BABY BOOM; WUSCHEL
资金
- Donald Danforth Plant Science Center
- Open Innovation Fund from Corteva Agriscience
Teff is a staple food and cash crop in Ethiopia, but lodging has been a major limitation to its production. Using CRISPR/Cas9, researchers introduced mutations in the tef orthologue of the SD-1 gene to confer semidwarfism and lodging resistance. By delivering gene editing reagents along with transformation enhancing genes, they achieved high frequency recovery of transgenic and edited tef lines. These lines showed reduced culm and internode lengths and increased lodging resistance, highlighting the potential of genome editing in improving important traits in tef.
Tef is a staple food and a valuable cash crop for millions of people in Ethiopia. Lodging is a major limitation to tef production, and for decades, the development of lodging resistant varieties proved difficult with conventional breeding approaches. We used CRISPR/Cas9 to introduce knockout mutations in the tef orthologue of the rice SEMIDWARF-1 (SD-1) gene to confer semidwarfism and ultimately lodging resistance. High frequency recovery of transgenic and SD-1 edited tef lines was achieved in two tef cultivars by Agrobacterium-mediated delivery into young leaf explants of gene editing reagents along with transformation and regeneration enhancing morphogenic genes, BABY BOOM (BBM) and WUSCHEL2 (WUS2). All of the 23 lines analyzed by next-generation sequencing had at least two or more alleles of SD-1 mutated. Of these, 83% had tetra-allelic frameshift mutations in the SD-1 gene in primary tef regenerants, which were inherited in subsequent generations. Phenotypic data generated on T-1 and T-2 generations revealed that the sd-1 lines have reduced culm and internode lengths with no reduction in either panicle or peduncle lengths. These characteristics are comparable with rice sd-1 plants. Measurements of lodging, in greenhouse-grown plants, showed that sd-1 lines have significantly higher resistance to lodging at the heading stage compared with the controls. This is the first demonstration of the feasibility of high frequency genetic transformation and CRISPR/Cas9-mediated genome editing in this highly valuable but neglected crop. The findings reported here highlight the potential of genome editing for the improvement of lodging resistance and other important traits in tef.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据