期刊
PLANT AND CELL PHYSIOLOGY
卷 63, 期 10, 页码 1433-1445出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcac043
关键词
Cyanobacteria; Differential proteomic; Nitrogen; Rice; Symbiosis
资金
- Fundacion General Consejo Superior de Investigaciones Cientificas (Programa ComFuturo)
- UE [US-1380747]
This study analyzed the protein changes in rice and nostoc during the early stages of symbiosis using mass spectrometry, and identified differentially expressed proteins related to signal transduction, adhesion, defense, and cell wall modification. The study also revealed new proteins activated during the early stages of the nostoc-rice symbiosis. These findings provide important insights into the symbiotic interaction between nostoc and land plants.
Symbiosis between cyanobacteria and plants is considered pivotal for biological nitrogen deposition in terrestrial ecosystems. Despite extensive knowledge of the ecology of plant-cyanobacterium symbioses, little is known about the molecular mechanisms involved in recognition between partners. Here, we conducted a quantitative sequential window acquisition of all theoretical fragment ion spectra mass spectrometry pipeline to analyze protein changes in Oryza sativa and Nostoc punctiforme during early events of symbiosis. We found differentially expressed proteins in both organisms linked to several biological functions, including signal transduction, adhesion, defense-related proteins and cell wall modification. In N. punctiforme we found increased expression of 62 proteins that have been previously described in other Nostoc-plant symbioses, reinforcing the robustness of our study. Our findings reveal new proteins activated in the early stages of the Nostoc-Oryza symbiosis that might be important for the recognition between the plant and the host. Oryza mutants in genes in the common symbiosis signaling pathway (CSSP) show reduced colonization efficiency, providing first insights on the involvement of the CSSP for the accommodation of N. punctiforme inside the plant cells. This information may have long-term implications for a greater understanding of the symbiotic interaction between Nostoc and land plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据