Two new polyhydroxylated steroidal glycosides from Paris polyphylla var. yunnanensis

Chemical investigation of Paris polyphylla smith var. yunnanensis afforded two new polyhydroxylated steroidal glycosides, named Parisyunnanosides K and L (1-2), together with nine known ones. The chemical structures of the new compounds were elucidated by 1D, 2D NMR and HR-ESI-MS techniques, together with chemical methods. Parisyunnanosides K and L (1-2) are rare C-27 steroidal glycosides with two double bonds located at C-5, 6 and C-25, 26 of the aglycone, respectively. The cytotoxic activities of the isolated compounds were evaluated against Caco-2 cells by CCK-8 assay. Among them, compounds 5-11 exhibited potent cytotoxic activity with IC50 values ranging from 1.10 to 14.14 mu M compared with the positive control oxaliplatin (1.38 mu M).

Automated summary

Chemical investigation of Paris polyphylla smith var. yunnanensis led to the discovery of two new polyhydroxylated steroidal glycosides, named Parisyunnanosides K and L (1-2), along with nine known compounds. The new compounds, Parisyunnanosides K and L (1-2), are rare C-27 steroidal glycosides. Furthermore, cytotoxic activity evaluation revealed that compounds 5-11 exhibited potent cytotoxic activity.