Gastroprotective effects of water extract of domesticated Amauroderma rugosum against several gastric ulcer models in rats

Context: Amauroderma rugosum (Blume & T. Nees) Torrend (Ganodermataceae) is an edible mushroom with medicinal properties. However, the effects of A. rugosum on gastric ulcer remain unclear. Objective: To investigate the gastroprotective efficacy of water extract of A. rugosum (WEA) on gastric ulcer. Materials and methods: Sprague-Dawley rats were randomly grouped as control, model, lansoprazole and 200, 100 and 50 mg/kg of WEA. After pre-treatment for seven days, ethanol- and indomethacin-induced gastric ulcer models were established. The gastric ulcer and histopathology were investigated. Enzyme-linked immunosorbent assay (ELISA), quantitative polymerase chain reaction (Q-PCR) and Western blot assays were conducted to explore the potential anti-inflammatory effect and mechanism of WEA. Additionally, the pyloric ligation model was used to explore the influence of WEA on gastric acid and mucus. Results: Pre-treatment with WEA (200, 100 and 50 mg/kg) effectively reduced ulcerous area in both ethanol-induced (71%, 88% and 71%) and indomethacin-induced (77%, 65% and 86%) gastric ulcer model. The gastric levels of tumour necrosis factor-alpha (TNF-alpha) (34% and 50 mg/kg), interleukin-6 (IL-6) (32% and 100 mg/kg) and interleukin-1 beta (IL-1 beta) (36%, 45% and 41%) were reduced significantly (p < 0.05) by WEA. Serum nitric oxide was decreased significantly (p < 0.05) at 200 and 50 mg/kg and PGE2 concentration was increased remarkably (p < 0.05) at 100mg/kg. Gene expression of inflammasome Nlrp3, and the nuclear translocation of nuclear factor-kappa B (NF-kappa B) P65 were significantly decreased by WEA pre-treatment. However, the pH of gastric acid and secretion of mucus did not show any significant change. Conclusions: The gastroprotective effect of WEA on gastric damage is attributed to anti-inflammation through the inhibition on NF-kappa B P65 nuclear migration and Nlrp3 gene expression.

Automated summary

The water extract of A. rugosum (WEA) has been found to have gastroprotective effects, reducing the size of gastric ulcers and exhibiting anti-inflammatory properties. Pre-treatment with WEA decreased the concentration of inflammatory factors in the stomach and did not have a significant impact on gastric acid and mucus secretion.