期刊
PEST MANAGEMENT SCIENCE
卷 78, 期 8, 页码 3540-3550出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.6997
关键词
dopamine; microRNA; TH; metamorphosis; Helicoverpa armigera
资金
- National Natural Science Foundation of China [31972275]
The study investigates the regulatory functions of dopamine (DA) and its associated non-coding RNA in the metamorphosis of the cotton bollworm, Helicoverpa armigera. It is found that DA deficiency inhibits larval-pupal metamorphosis in H. armigera. The study suggests that DA pathway genes and their microRNAs could be used as a novel target for H. armigera management.
BACKGROUND The cotton bollworm, Helicoverpa armigera, is a worldwide polyphagous pest, causing huge economic losses in vegetable, cotton and corn crops, among others. Owing to long-term exposure to Bacillus thuringiensis (Bt) toxins, evolution of resistance has been detected in this pest. As a conservative and effective neurotransmitter, dopamine (DA) has an important role in insect growth and development. In this study, we investigated the regulatory functions of DA and its associated non-coding RNA in metamorphosis in H. armigera. RESULTS Expression profiles indicated that DA and DA pathway genes were highly expressed during larval-pupal metamorphosis in H. armigera. RNA interference and pharmacological experiments confirmed that tyrosine hydroxylase (TH), dopa decarboxylase, vesicular amine transporter and DA receptor 2 are critical genes related to the development of H. armigera from larvae to pupae. We also found that miR-14 and miR-2766 targeted the 3 ' untranslated region to post-transcriptionally regulate HaTH function. Application of miR-2766 and miR-14 antagomirs significantly increased levels of HaTH transcripts and proteins, while injection of miR-2766 and miR-14 agomirs not only suppressed messenger RNA and protein levels of HaTH, but also resulted in defective pupation in H. armigera. CONCLUSION These results suggest that DA deficiency inhibits larval-pupal metamorphosis in H. armigera. Potentially, DA pathway genes and their microRNAs could be used as a novel target for H. armigera management. (c) 2022 Society of Chemical Industry.
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