Glutamicibacter halophytocola-mediated host fitness of potato tuber moth on Solanaceae crops

BACKGROUND: The potato tuber moth (PTM), Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is a destructive pest of Solanaceae crops worldwide. alpha-solanine and alpha-chaconine are toxic steroidal glycoalkaloids (SGAs) in Solanaceae crops and are most abundant in potatoes (Solanum tuberosum L.), accounting for more than 95% of the total SGAs. PTM grows on potatoes with a higher concentration of SGAs. Gut bacteria play an important role in the physiology and behavior of insects. To understand the role of gut bacteria of PTM in host adaptability, we isolated and identified major SGA (alpha-chaconine and alpha-solanine)-degrading gut bacteria in the gut of PTM by a selective medium and analyzed their degradability and degradation mechanism. RESULTS: The gut Glutamicibacter halophytocola S2 of PTM with high degradation capacity to alpha-solanine and alpha-chaconine were detected by liquid chromatography mass spectrometry (LC-MS) and identified by morphological and 16S rRNA gene sequence analysis. A gene cluster involving alpha-rhamnosidases, beta-glucosidases, and beta-galactosidases was identified by whole-genome sequencing of G. halophytocola S2. These genes had higher expression on the alpha-solanine medium. PTM inoculated with the isolated G. halophytocola S2 obtained higher fitness than antibiotic-treated PTM. CONCLUSION: The G. halophytocola S2 in the gut of PTM could degrade the major toxic alpha-solanine and alpha-chaconine in potatoes. This enhances the fitness of PTM feeding on potatoes with high SGA contents. The results provide a theoretical foundation for the integrated pest management of PTM and provide an effective strain for the treatment of alpha-solanine and alpha-chaconine in potato food. (C) 2022 Society of Chemical Industry.

Automated summary

It has been discovered that the gut bacteria Glutamicibacter halophytocola S2 in the potato tuber moth can degrade the toxic substances alpha-solanine and alpha-chaconine in potatoes. This enhances the adaptability of the moth on its host, potatoes.