Laodelphax striatellus saliva mucin enables the formation of stylet sheathes to facilitate its feeding and rice stripe virus transmission

Background Laodelphax striatellus transmits rice stripe virus (RSV) during sap feeding on the rice plant. The insect saliva proteins have direct and indirect roles in mediating RSV transmission; however, the function of most saliva proteins remains unclear. Results In this study, we sequenced L. striatellus saliva proteins using shotgun liquid chromatography-electrospray ionization-tandem mass spectrometry. We identified 41 secreted saliva proteins, among which a saliva mucin-like protein, designated LssaMP, was the most abundant. In silico analysis revealed the sequence conservation among planthoppers. We revealed that the LssaMP gene is specifically expressed in the salivary glands and the protein is secreted as a component of gel saliva. Using LssaMP-specific double-stranded RNA (dsRNA) to silence gene expression, we revealed that LssaMP is required for formation of the salivary sheath, an important structure for sap feeding. Disrupting LssaMP expression resulted in inefficient formation of the feeding structure, thereby stopping insects from secreting watery saliva and acquiring sufficient nutrients from the phloem sap. We confirmed that RSV is mainly released via the watery saliva, which passes through the salivary sheathes into the plant phloem. An insufficient feeding structure results in decreased release of watery saliva, as well as the arboviruses. Conclusion This study clarified the function of an insect saliva protein in mediating insect feeding, as well as arbovirus transmission. (c) 2022 Society of Chemical Industry.

Automated summary

This study elucidated the function of an insect saliva protein in mediating insect feeding and arbovirus transmission by sequencing the saliva proteins of Laodelphax striatellus. The saliva protein was found to be involved in the formation of the salivary sheath, which is crucial for sap feeding and viral transmission.