Prevalence and genetic characterization of Enterocytozoon bieneusi in children in Northeast Egypt

Enterocytozoon bieneusi is the most common microsporidia in humans worldwide, in addition to infecting a wide range of animals. However, there is limited information about this pathogen in children in Egypt. Here, we carried out a molecular epidemiological study of E. bieneusi in child care centers in three provinces in Egypt. Altogether, 585 fresh fecal samples were collected from children attending 18 child care centers in El-Dakahlia, El-Gharbia, and Damietta provinces in Northeast Egypt during March 2015 to April 2016. PCR and sequence analyses of the ribosomal internal transcribed spacer (ITS) were used to detect and genotype E. bieneusi. Twenty-seven fecal samples (4.6%, 27/585) were positive for E. bieneusi. Five genotypes were identified, including type IV (n= 13), Peru8 (n= 9), Peru6 (n = 2), Perull (n= 2), and D (n= 1). Phylogenetic analysis indicated that the five genotypes of E. bieneusi detected in this study were clustered into zoonotic group 1. These data provide important information on the prevalence and genetic diversity of E. bieneusi in children in this country. Further epidemiological studies should be conducted to elucidate the role of zoonotic transmission in human E. bieneusi infections.

Automated summary

This study conducted a molecular epidemiological study of E. bieneusi in child care centers in Egypt. The results showed that the infection rate of E. bieneusi was 4.6% among children in these centers, and five genotypes were identified. These data provide important information on the epidemiology and genetic diversity of E. bieneusi in children in Egypt.