4.8 Article

hnRNP G/RBMX enhances HPV16 E2 mRNA splicing through a novel splicing enhancer and inhibits production of spliced E7 oncogene mRNAs

期刊

NUCLEIC ACIDS RESEARCH
卷 50, 期 7, 页码 3867-3891

出版社

OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac213

关键词

-

资金

  1. Swedish Research Council-Medicine [VR2019-01210]
  2. Swedish Cancer Society [CAN2018/702]
  3. China Scholarship Council [201706170061, 201606525004, 201809120016]
  4. Swedish Research Council [VR2019-01210]

向作者/读者索取更多资源

By investigating the expression regulation of Human papillomavirus type 16 (HPV16) E2, a required splicing enhancer has been identified, which interacts with cellular RNA binding protein hnRNP G and promotes splicing and production of E2 mRNA. The splicing-enhancing function of hnRNP G is mediated by its interaction with splicing factor U2AF65. The interaction between hnRNP G and HPV16 E2 mRNA and U2AF65 is increased by keratinocyte differentiation and DNA damage response, while sumoylation of hnRNP G is reduced under the DNA damage response, leading to increased splicing and interactions between hnRNP G and E2 mRNA as well as U2AF65. Moreover, hnRNP G inhibits the splicing of the HPV16 E6 coding region, thereby suppressing the production of spliced E7 oncogene mRNAs.
Human papillomavirus type 16 (HPV16) E2 is an essential HPV16 protein. We have investigated how HPV16 E2 expression is regulated and have identifed a splicing enhancer that is required for production of HPV16 E2 mRNAs. This uridine-less splicing enhancer sequence (ACGAGGACGAGGACAAGGA) contains 84% adenosine and guanosine and 16% cytosine and consists of three 'AC(A/G)AGG'-repeats. Mutational inactivation of the splicing enhancer reduced splicing to E2-mRNA specific splice site SA2709 and resulted in increased levels of unspliced E1-encoding mRNAs. The splicing enhancer sequence interacted with cellular RNA binding protein hnRNP G that promoted splicing to SA2709 and enhanced E2 mRNA production. The splicing-enhancing function of hnRNP G mapped to amino acids 236-286 of hnRNP G that were also shown to interact with splicing factor U2AF65. The interactions between hnRNP G and HPV16 E2 mRNAs and U2AF65 increased in response to keratinocyte differentiation as well as by the induction of the DNA damage response (DDR). The DDR reduced sumoylation of hnRNP G and pharmacological inhibition of sumoylation enhanced HPV16 E2 mRNA splicing and interactions between hnRNP G and E2 mRNAs and U2AF65. Intriguingly, hnRNP G also promoted intron retention of the HPV16 E6 coding region thereby inhibiting production of spliced E7 oncogene mRNAs.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据