期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 29, 期 6, 页码 529-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41594-022-00774-y
关键词
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资金
- USPHS National Research Service Award [5T32GM008496]
- UCLA-Caltech Medical Scientist Training Program
- HHMI
- NIH [GM123126, AG 054022, AG 048120]
- National Institute of General Medical Sciences from the National Institutes of Health [P30 GM124165]
- NIH-ORIP HEI grant [S10OD021527]
- DOE [DE-FC02-02ER63421]
- DOE Office of Science [DE-AC02-06CH11357]
Mutations in the low-complexity domains (LCDs) of disease-associated proteins can promote the formation of amyloid-like aggregates, leading to disease. In this study, mutations in the intermediate filament protein KRT8 were found to promote amyloid formation and were observed in hepatocytes of livers with alcoholic steatohepatitis (ASH). Ethanol was shown to further promote KRT8 aggregation, and the aggregated KRT8 was found to co-crystallize with alcohol. Liver extract from people with ASH could also seed KRT8 aggregation, indicating the amyloid nature of KRT8 aggregates and the association of ASH with amyloid-related conditions.
Proteins including FUS, hnRNPA2, and TDP-43 reversibly aggregate into amyloid-like fibrils through interactions of their low-complexity domains (LCDs). Mutations in LCDs can promote irreversible amyloid aggregation and disease. We introduce a computational approach to identify mutations in LCDs of disease-associated proteins predicted to increase propensity for amyloid aggregation. We identify several disease-related mutations in the intermediate filament protein keratin-8 (KRT8). Atomic structures of wild-type and mutant KRT8 segments confirm the transition to a pleated strand capable of amyloid formation. Biochemical analysis reveals KRT8 forms amyloid aggregates, and the identified mutations promote aggregation. Aggregated KRT8 is found in Mallory-Denk bodies, observed in hepatocytes of livers with alcoholic steatohepatitis (ASH). We demonstrate that ethanol promotes KRT8 aggregation, and KRT8 amyloids co-crystallize with alcohol. Lastly, KRT8 aggregation can be seeded by liver extract from people with ASH, consistent with the amyloid nature of KRT8 aggregates and the classification of ASH as an amyloid-related condition.
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