期刊
NATURE CHEMICAL BIOLOGY
卷 18, 期 7, 页码 698-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41589-022-00992-x
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资金
- National Institutes of Health (NIH) [T32 GM067543, T32 AI007281]
- Gruber Science Fellowship
- NSF [DGE 1147470]
- NIH [AI118932, CA116087, R35GM134964, R35GM137952]
- Department of Veterans Affairs [BX004447]
- Novo Nordisk Foundation Challenge Programme [NNF19OC0056411]
- American Cancer Society Institutional Research Grant [IRG 17-172-57]
- Yale Cancer Center
- Conquer Cancer Now Award from the Concern Foundation
- NIH Research Grant from the National Cancer Institute [CA-16359]
- Stanford Graduate Fellowship
Oxidative stress, a prominent characteristic of most cancers, is involved in tumor formation. However, the molecular events of oxidation contributing to tumorigenesis remain largely unknown. This study demonstrates that inactivation of a redox-sensitive cysteine in the host protease legumain, which is oxidized during infection with the gastric cancer-causing bacterium Helicobacter pylori, accelerates tumor growth. These findings establish a connection between infection-induced oxidation and tumorigenesis, highlighting the significance of cysteine reactivity in tumor growth.
Oxidative stress is a defining feature of most cancers, including those that stem from carcinogenic infections. Reactive oxygen species can drive tumor formation, yet the molecular oxidation events that contribute to tumorigenesis are largely unknown. Here we show that inactivation of a single, redox-sensitive cysteine in the host protease legumain, which is oxidized during infection with the gastric cancer-causing bacterium Helicobacter pylori, accelerates tumor growth. By using chemical proteomics to map cysteine reactivity in human gastric cells, we determined that H. pylori infection induces oxidation of legumain at Cys219. Legumain oxidation dysregulates intracellular legumain processing and decreases the activity of the enzyme in H. pylori-infected cells. We further show that the site-specific loss of Cys219 reactivity increases tumor growth and mortality in a xenograft model. Our findings establish a link between an infection-induced oxidation site and tumorigenesis while underscoring the importance of cysteine reactivity in tumor growth.
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