Genetically encoded tools for measuring and manipulating metabolism

Over the past few years, we have seen an explosion of novel genetically encoded tools for measuring and manipulating metabolism in live cells and animals. Here, we will review the genetically encoded tools that are available, describe how these tools can be used and outline areas where future development is needed in this fast-paced field. We will focus on tools for direct measurement and manipulation of metabolites. Metabolites are master regulators of metabolism and physiology through their action on metabolic enzymes, signaling enzymes, ion channels and transcription factors, among others. We hope that this Perspective will encourage more people to use these novel reagents or even join this exciting new field to develop novel tools for measuring and manipulating metabolism.

Automated summary

In recent years, there has been a rapid development of novel genetically encoded tools for measuring and manipulating metabolism in live cells and animals. This article reviews the available genetically encoded tools, describes their usage, and outlines future directions for development. The focus is on tools for directly measuring and manipulating metabolites, which are key regulators of metabolism and physiology through their action on enzymes, channels, and factors. The aim of this Perspective is to encourage more individuals to utilize these new tools and potentially engage in the development of novel tools for measuring and manipulating metabolism.