期刊
NATURE
卷 606, 期 7915, 页码 761-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-022-04835-6
关键词
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资金
- Telethon [TGM16CBDM13, TGM16CBDM09]
- Italian Association for Cancer Research [IG2013_14761, 25174]
- European Research Council [670881]
- University of Naples Federico II
- Italian Ministry of University and Research (PRIN, 2020PKLEPN)
- Inova program Fiocruz
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- European Research Council (ERC) [670881] Funding Source: European Research Council (ERC)
The replication organelle of SARS-CoV-2 is generated by viral non-structural proteins (NSPs) NSP3 and NSP4, while NSP6 plays a role in connecting ER membranes and organizing DMV clusters. NSP6 also mediates the contact between SARS-CoV-2 and lipid droplets (LDs), providing a means to refurbish the replication organelle with LD-derived lipids.
SARS-CoV-2, like other coronaviruses, builds a membrane-bound replication organelle to enable RNA replication(1). The SARS-CoV-2 replication organelle is composed of double-membrane vesicles (DMVs) that are tethered to the endoplasmic reticulum (ER) by thin membrane connectors(2), but the viral proteins and the host factors involved remain unknown. Here we identify the viral non-structural proteins (NSPs) that generate the SARS-CoV-2 replication organelle. NSP3 and NSP4 generate the DMVs, whereas NSP6, through oligomerization and an amphipathic helix, zippers ER membranes and establishes the connectors. The NSP6(Delta SGF) mutant, which arose independently in the Alpha, Beta, Gamma, Eta, Iota and Lambda variants of SARS-CoV-2, behaves as a gain-of-function mutant with a higher ER-zippering activity. We identified three main roles for NSP6: first, to act as a filter in communication between the replication organelle and the ER, by allowing lipid flow but restricting the access of ER luminal proteins to the DMVs; second, to position and organize DMV clusters; and third, to mediate contact with lipid droplets (LDs) through the LD-tethering complex DFCP1-RAB18. NSP6 thus acts as an organizer of DMV clusters and can provide a selective means of refurbishing them with LD-derived lipids. Notably, both properly formed NSP6 connectors and LDs are required for the replication of SARS-CoV-2. Our findings provide insight into the biological activity of NSP6 of SARS-CoV-2 and of other coronaviruses, and have the potential to fuel the search for broad antiviral agents.
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