期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 72, 期 1, 页码 99-103出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkw353
关键词
-
资金
- Health and Medical Research Fund of the Food and Health Bureau of the Hong Kong Special Administrative Region [HKM-15-M10]
Objectives: To characterize bla(IMP-4)-carrying plasmids originating from inpatients in Hong Kong. Methods: Sixteen blaIMP-4-carrying plasmids identified among Enterobacteriaceae (nine Escherichia coli, four Klebsiella pneumoniae, two Citrobacter freundii and one Enterobacter cloacae) recovered from 15 patients were characterized. The isolates, collected during January 2010 to December 2013, were retrospectively investigated by plasmid sequencing, molecular and fitness studies. Results: The blaIMP-4-carrying plasmids belonged to the IncN ST7 lineage (similar to 50kb). Twelve of the 16 plasmids were epidemiologically linked to seven different regions in China. Alignment of the complete plasmid sequences showed identical plasmid backbones and two highly similar resistance regions, each carrying one of two resistance genes (bla(IMP-4) and qnrS1). The blaIMP-4 was detected in a class 1 integron (containing blaIMP-4 and intron Kl. pn. 13) that is part of an IS6100-IS26 transposon-like structure. The nine E. coli carrying the epidemic plasmid belonged to multiple multilocus STs (six ST542, one ST131, one ST657 and one ST3177). Fitness assays performed on E. coli J53 recipients showed that the presence of the epidemic plasmid did not have a significant biological cost. Conclusions: This study identified a blaIMP-4-carrying IncN ST7 plasmid disseminated amongmultiple enterobacterial species originating from patients with epidemiological links to different regions in China.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据