期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 44, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.nano.2022.102569
关键词
beta-Amyloid; Gold nanorods; Surface enhanced fluorescence; CRANAD; C. elegans
资金
- Agencia Nacional de Investigacion y Desarrollo (ANID)
- FONDECYT [1201668, 1211482, 1190623, 1201962, 3180651, 15130011]
- FONDEF [ID21I10347 Millennium Science Initiative Program-ICN09_016/ICN 2021_045]
- CARE-Chile-UC Center [AFB170005, UST 34827, ACT210068]
- ANID [21200617]
- Fondecyt Posdoctorate [3200610, 77200057]
- PAI [3190547]
- Beca Doctorado Nacional ANID [21180739, 21221615]
- Fondequip [EQM170111, EQM160157]
This study demonstrates an improved detection of A beta aggregates in C. elegans and mouse models of AD by co-administering functionalized gold nanorods with fluorescent probes, suggesting the potential of using plasmonic gold nanoparticles to enhance the detection of A beta in vivo.
The beta-amyloid (A beta) peptide is one of the key etiological agents in Alzheimer's disease (AD). The in vivo detection of A beta species is challenging in all stages of the illness. Currently, the development of fluorescent probes allows the detection of A beta in animal models in the near-infrared region (NIR). However, considering future applications in biomedicine, it is relevant to develop strategies to improve detection of amyloid aggregates using NIR probes. An innovative approach to increase the fluorescence signal of these fluorophores is the use of plasmonic gold nanoparticles (surface-enhanced fluorescence effect). In this work, we improved the detection of A beta aggregates in C. elegans and mouse models of AD by co-administering functionalized gold nanorods (GNRs-PEG-D1) with the fluorescent probes CRANAD-2 or CRANAD-58, which bind selectively to different amyloid species (soluble and insoluble). This work shows that GNRs improve the detection of A beta using NIR probes in vivo. (C) 2022 Published by Elsevier Inc.
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