Optical Quantification by Nanopores of Viruses, Extracellular Vesicles, and Nanoparticles

Nanopores combined with optical approaches canbe used to detect viral particles. In this work, we demonstrate theability of hydrodynamical driving and optical sensing to identifyand quantify viral particles in a biological sample. We havedeveloped a simple and rapid method which requires onlyfluorescent labeling of the particles and can therefore be appliedto a wide range of virus type. The system operates in real time andat the single particle level while providing a low error onconcentration (4%) and a low limit of detection of 105particles/mL for an acquisition time of 60 s with the ability to increase the acquisition time to achieve a lower limit

Automated summary

This study demonstrates the capability of combining hydrodynamical driving and optical sensing to identify and quantify viral particles in a biological sample. The method is simple and rapid, requiring only fluorescent labeling of the particles, and can be applied to a wide range of virus types. The system operates in real time and provides low concentration error and limit of detection at the single particle level.