期刊
MOLECULES
卷 27, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/molecules27061963
关键词
volatile organic compounds (VOCs); headspace solid-phase microextraction (HS-SPME); SPME-GC-MS; stored-product insect pests; Tribolium castaneum; Rhyzopertha dominica; Sitophilus granarius
资金
- Civil Services Bureau of Fujian Province for Early Career Researcher [132120030]
- Australian Research Council Discovery Early Career Researcher Award (DECRA) [DE160100382]
Monitoring and early detection of stored-grain insect infestation is crucial for effective pest management. This study presents a reliable method using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry to assess insect infestation in stored grains. Specific volatile compounds emitted by the infested grains were identified and can be utilized for insect surveillance and monitoring in stored grain.
Monitoring and early detection of stored-grain insect infestation is essential to implement timely and effective pest management decisions to protect stored grains. We report a reliable analytical procedure based on headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) to assess stored-grain infestation through the detection of volatile compounds emitted by insects. Four different fibre coatings were assessed; 85 mu m CAR/PDMS had optimal efficiency in the extraction of analytes from wheat. The headspace profiles of volatile compounds produced by Tribolium castaneum (Herbst), Rhyzopertha dominica (Fabricius), and Sitophilus granarius (Linnaeus), either alone or with wheat, were compared with those of non-infested wheat grains. Qualitative analysis of chromatograms showed the presence of different volatile compound profiles in wheat with pest infestation compared with the wheat controls. Wheat-specific and insect-specific volatile compounds were identified, including the aggregation pheromones, dominicalure-1 and dominicalure-2, from R. dominica, and benzoquinones homologs from T. castaneum. For the first time, the presence of 3-hydroxy-2-butanone was reported from S. granarius, which might function as an alarm pheromone. These identified candidate biomarker compounds can be utilized in insect surveillance and monitoring in stored grain to safeguard our grain products in future.
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