期刊
MOLECULES
卷 27, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/molecules27061940
关键词
bryophytes; mosses; Dicranum majus; Thuidium delicatulum; anti-inflammatory activity; nitric oxide
资金
- Marie Sklodowska Actions Innovative Training Networks under the Horizon 2020 program [765115-MossTech]
- Impact Biomolecules project of the Lorraine Universite d'Excellence (Investissements d'avenir-ANR)
- fond regional de cooperation pour la recherche Grand Est, (Biomolecules et Biomateriaux pour la Bioeconomie Regionale 3BR project)
The study found that the biologically active extracts of Dicranum majus and Thuidium delicatulum exhibited significant anti-inflammatory effects, potentially serving as a perspective for developing innovative therapeutic agents.
Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of Dicranum majus and Thuidium delicatulum inhibited NO production in a concentration-dependent manner with IC50 values of 1.04 and 1.54 mu g/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据