期刊
MOLECULES
卷 27, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/molecules27051549
关键词
fraxinol; B16-F10 cells; melanogenesis; microphthalmia-associated transcription factor; depigmentation
资金
- National Research Foundation (NRF) of Korea [NRF-2018R1C1B6007728, NRF-2021R1C1C1006516]
Researchers have found that fraxinol, a natural coumarin derived from Fraxinus plants, can effectively stimulate melanogenesis, which may have potential value in the treatment of chronic depigmentation conditions such as vitiligo.
Melanin pigment produced in melanocytes plays a protective role against ultraviolet radiation. Selective destruction of melanocytes causes chronic depigmentation conditions such as vitiligo, for which there are very few specific medical treatments. Here, we found that fraxinol, a natural coumarin from Fraxinus plants, effectively stimulated melanogenesis. Treatment of B16-F10 cells with fraxinol increased the melanin content and tyrosinase activity in a concentration-dependent manner without causing cytotoxicity. Additionally, fraxinol enhanced the mRNA expression of melanogenic enzymes such as tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. Fraxinol also increased the expression of microphthalmia-associated transcription factor at both mRNA and protein levels. Fraxinol upregulated the phosphorylation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB). Furthermore, H89, a cAMP-dependent protein kinase A inhibitor, decreased fraxinol-induced CREB phosphorylation and microphthalmia-associated transcription factor expression and significantly attenuated the fraxinol-induced melanin content and intracellular tyrosinase activity. These results suggest that fraxinol enhances melanogenesis via a protein kinase A-mediated mechanism, which may be useful for developing potent melanogenesis stimulators.
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