4.7 Article

A secreted ribonuclease effector from Verticillium dahliae localizes in the plant nucleus to modulate host immunity

期刊

MOLECULAR PLANT PATHOLOGY
卷 23, 期 8, 页码 1122-1140

出版社

WILEY
DOI: 10.1111/mpp.13213

关键词

host immunity; secreted ribonuclease; VdRTX1; Verticillium dahliae

资金

  1. National Key Research and Development Program of China [2018YFE0112500]
  2. National Natural Science Foundation of China [31972228, 31970142, 31870138]
  3. Fundamental Research Funds for Central Non-profit Scientific Institution in CAAS [Y2021XK22, Y2018PT70]

向作者/读者索取更多资源

The study reveals that a ribonuclease secreted by Verticillium dahliae, VdRTX1, can translocate into the plant nucleus to modulate plant immunity. VdRTX1 induces cell death and possesses specific functions that are not present in other fungi.
The arms race between fungal pathogens and plant hosts involves recognition of fungal effectors to induce host immunity. Although various fungal effectors have been identified, the effector functions of ribonucleases are largely unknown. Herein, we identified a ribonuclease secreted by Verticillium dahliae (VdRTX1) that translocates into the plant nucleus to modulate immunity. The activity of VdRTX1 causes hypersensitive response (HR)-related cell death in Nicotiana benthamiana and cotton. VdRTX1 possesses a signal peptide but is unlikely to be an apoplastic effector because its nuclear localization in the plant is necessary for cell death induction. Knockout of VdRTX1 significantly enhanced V. dahliae virulence on tobacco while V. dahliae employs the known suppressor VdCBM1 to escape the immunity induced by VdRTX1. VdRTX1 homologs are widely distributed in fungi but transient expression of 24 homologs from other fungi did not yield cell death induction, suggesting that this function is specific to the VdRTX1 in V. dahliae. Expression of site-directed mutants of VdRTX1 in N. benthamiana leaves revealed conserved ligand-binding sites that are important for VdRTX1 function in inducing cell death. Thus, VdRTX1 functions as a unique HR-inducing effector in V. dahliae that contributes to the activation of plant immunity.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据