期刊
MOLECULAR PHARMACEUTICS
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.molpharmaceut.2c00070
关键词
prostate cancer; breast cancer; MD simulations; PSMA; GRPR; PET imaging; PC-3; LNCaP; T47D; MD-MB231
Prostate-specific membrane antigen (PSMA) and gastrin-releasing peptide receptor (GRPR) have been used as targets for molecular imaging and therapy of prostate and breast cancer. The study synthesized and tested three bioconjugate probes and found that PSMA overexpression resulted in increased binding of the probes to PCa and BCa cells, especially in those already expressing GRPR. Biomolecular simulations provided valuable information about the molecular mechanisms involved in the interactions of the probes with PSMA and GRPR.
Prostate-specific membrane antigen (PSMA) and gastrin-releasing peptide receptor (GRPR) have both been used in nuclear medicine as targets for molecular imaging and therapy of prostate (PCa) and breast cancer (BCa). Three bioconjugate probes, the PSMA specific: [Ga-68]Ga-1, ((HBED-CC)-Ahx-Lys-NH-CO-NH Glu or PSMA-11), the GRPR specific: [Ga-68]Ga-2, ((HBED-CC)-4-amino-1-carboxymethyl pip eridine-[D-Phe(6), Sta(13)]BN(6-14), a bombesin (BN) analogue), and 3 (the BN analogue: 4-amino-1-carboxymethyl piperidine-[(R)-Phe(6), Sta(13)]-BN(6-14) connected with the fluorescent dye, BDP-FL), were synthesized and tested in vitro with PCa and BCa cell lines, more specifically, with PCa cells, PC-3 and LNCaP, with BCa cells, T47D, MDA-MB-231, and with the in-house created PSMA-overexpressing PC-3((PSMA)), T47D((PSMA)), and MDA-MB-231((PSMA)). In addition, biomolecular simulations were conducted on the association of 1 and 2 with PSMA and GRPR. The PSMA overexpression resulted in an increase of cell-bound radioligand [Ga-68]Ga-1 (PSMA) for PCa and BCa cells and also of [Ga-68]Ga-2 (GRPR), especially in those cell lines already expressing GRPR. The results were confirmed by fluorescence-activated cell sorting with a PE-labeled PSMA-specific antibody and the fluorescence tracer 3. The docking calculations and molecular dynamics simulations showed how 1 enters the PSMA funnel region and how pharmacophore Glu-urea-Lys interacts with the arginine patch, the S1 & PRIME;, and S1 subpockets by forming hydrogen and van der Waals bonds. The chelating moiety of 1, that is, HBED-CC, forms additional stabilizing hydrogen bonding and van der Waals interactions in the arene-binding site. Ligand 2 is diving into the GRPR transmembrane (TM) helical cavity, thereby forming hydrogen bonds through its amidated end, water-mediated hydrogen bonds, and pi-pi interactions. Our results provide valuable information regarding the molecular mechanisms involved in the interactions of 1 and 2 with PSMA and GRPR, which might be useful for the diagnostic imaging and therapy of PCa and BCa.
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