期刊
MOLECULAR ECOLOGY RESOURCES
卷 22, 期 6, 页码 2158-2170出版社
WILEY
DOI: 10.1111/1755-0998.13604
关键词
3D-printing; DNA adsorption; environmental DNA; hydroxyapatite; passive sampling
资金
- Office Francaise de la Biodiversite (OFB)
- Agence Francaise de la Recherche Technologique (ANRT)
- CNRS Mission pour les Initiatives Transverses et Interdisciplinaires (project XLIFE CAPTAS)
- Graduate School H2O'Lyon [ANR-17-EURE-0018]
- Eurofins Hydrobiologie France
- Universite de Lyon (UdL)
The study presents a concept validation of a passive, 3D-printed and easy-to-use eDNA sampler. The samplers, made from hydroxyapatite, efficiently collect genomic DNA and animal eDNA, but with variations in the amount of DNA collected. Further research on the DNA-hydroxyapatite interactions is needed to optimize eDNA adsorption and develop a reliable, easy-to-use, and reusable eDNA sampling tool.
The study of environmental DNA (eDNA) released by aquatic organisms in their habitat offers a fast, noninvasive and sensitive approach to monitor their presence. Common eDNA sampling methods such as water filtration and DNA precipitation are time-consuming, require difficult-to-handle equipment and partially integrate eDNA signals. To overcome these limitations, we created the first proof of concept of a passive, 3D-printed and easy-to-use eDNA sampler. We designed the samplers from hydroxyapatite (HAp samplers), a natural mineral with a high DNA adsorption capacity. The porous structure and shape of the samplers were designed to optimize DNA adsorption and facilitate their handling in the laboratory and in the field. Here we show that HAp samplers can efficiently collect genomic DNA in controlled set-ups, but can also collect animal eDNA under controlled and natural conditions with yields similar to conventional methods. However, we also observed large variations in the amount of DNA collected even under controlled conditions. A better understanding of the DNA-hydroxyapatite interactions on the surface of the samplers is now necessary to optimize eDNA adsorption and to allow the development of a reliable, easy-to-use and reusable eDNA sampling tool.
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