期刊
MOLECULAR ECOLOGY
卷 31, 期 12, 页码 3468-3480出版社
WILEY
DOI: 10.1111/mec.16476
关键词
macroalgae; marine disease; RNA-seq; seaweed; transcriptome
资金
- UNSW Sydney
- Australian Government Research Training Program Scholarship
This study used mRNA-sequencing analysis to investigate the early antipathogen response of the model macroalga Delisea pulchra under the environmental conditions that promote the onset of disease. The study identified unique transcripts affiliated with stress response and signal transduction processes, and revealed the downregulation of genes coding for predicted protein metabolism, stress response, energy generation, and photosynthesis functions in the presence of the opportunistic pathogen. This repression of core cellular processes likely interferes with the macroalgal antipathogen response, leading to infection, tissue damage, and bleaching symptoms.
Diseases in marine eukaryotic organisms caused by opportunistic pathogens represent a serious threat to our oceans with potential downstream consequences for ecosystem functioning. Disease outbreaks affecting macroalgae are of particular concern due to their critical role as habitat-forming organisms. However, there is limited understanding of the molecular strategies used by macroalgae to respond to opportunistic pathogens. In this study, we used mRNA-sequencing analysis to investigate the early antipathogen response of the model macroalga Delisea pulchra (Rhodophyta) under the environmental conditions that are known to promote the onset of disease. Using de novo assembly methods, 27,586 unique transcripts belonging to D. pulchra were identified that were mostly affiliated with stress response and signal transduction processes. Differential gene expression analysis between a treatment with the known opportunistic pathogen, Aquimarina sp. AD1 (Bacteroidota), and a closely related benign strain (Aquimarina sp. AD10) revealed a downregulation of genes coding for predicted protein metabolism, stress response, energy generation and photosynthesis functions. The rapid repression of genes coding for core cellular processes is likely to interfere with the macroalgal antipathogen response, later leading to infection, tissue damage and bleaching symptoms. Overall, this study provides valuable insight into the genetic features of D. pulchra, highlighting potential antipathogen response mechanisms of macroalgae and contributing to an improved understanding of host-pathogen interactions in a changing environment.
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