期刊
MOLECULAR CANCER THERAPEUTICS
卷 21, 期 7, 页码 1184-1194出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1535-7163.MCT-21-0872
关键词
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类别
资金
- Cancer Center Support Grant (CCSG) [CA076292]
- Moffitt Foundation [CA076292, R01CA157664, R01CA124515, R01CA178687]
- Junior Scientist Research Partnership Award [CA076292]
- H. Lee Moffitt Cancer Center
- American Cancer Society Postdoctoral Fellowship
- [R01CA211913]
- [1K99CA266947-01]
- [T32CA009140]
The expression of certain olfactory receptors in solid tumors provides a potential therapeutic target for epithelial cancers. CAR T cells targeting the olfactory receptor OR2H1 demonstrated specific cytotoxic killing of tumor cells in vitro and in vivo. This study suggests that T cells redirected against OR2H1-expressing tumor cells may be a promising therapy for a broad range of epithelial cancers.
Although chimeric antigen receptor (CAR)-expressing T cells have proven success in hematologic malignancies, their effective-ness in solid tumors has been largely unsuccessful thus far. We found that some olfactory receptors are expressed in a variety of solid tumors of different histologic subtypes, with a limited pattern of expression in normal tissues. Quantification of OR2H1 expres-sion by qRT-PCR and Western blot analysis of 17 normal tissues, 82 ovarian cancers of various histologies, eight non-small cell lung cancers (NSCLCs), and 17 breast cancers demonstrated widespread OR2H1 expression in solid epithelial tumors with expression in normal human tissues limited to the testis. CAR T cells recognizing the extracellular domain of the olfactory receptor OR2H1 were generated with a targeting motif identified through the screening of aphage display library and demonstrated OR2H1-specific cytotoxic killing in vitro and in vivo, using tumor cells with spontaneous expression of variable OR2H1 levels. Importantly, recombinant OR2H1 IgG generated with the VH/VL sequences of the CAR construct specifically detected OR2H1 protein signal in 60 human lung cancers, 40 ovarian carcinomas, and 73 cholangiocarcinomas, at positivity rates comparable with mRNA expression and without OR2H1 staining in 58 normal tissues. CRISPR/Cas9-mediated ablation of OR2H1 confirmed targeting specificity of the CAR and the tumor-promoting role of OR2H1 in glucose metabolism. Therefore, T cells redirected against OR2H1-expressing tumor cells represent a promising therapy against a broad range of epithelial cancers, likely with an admissible toxicity profile.
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