期刊
MOLECULAR BIOTECHNOLOGY
卷 64, 期 9, 页码 947-957出版社
SPRINGERNATURE
DOI: 10.1007/s12033-022-00461-9
关键词
Chitooligosaccharides; Green production; Chitosanase; Penicillium oxalicum
资金
- National Key R&D Program of China [2018YFD0901104]
- National Natural Science Foundation of China [31800679, 31801575]
- China Postdoctoral Science Foundation [2018M642167]
- Fundamental Research Funds for the Central Universities Institute of Food Science and Technology International Journal of Food Science Technology [JUSRP121008]
- China Agriculture Research System of MOF
- China Agriculture Research System of MARA
This study discovered a novel chitosanase from Penicillium oxalicum M2 and characterized its catalytic activity, stability, and substrate specificity. The enzyme showed high catalytic activity and stability within a certain temperature and pH range. It displayed strict specificity towards chitosan and could be activated by certain substances.
This study discovered a novel chitosanase from Penicillium oxalicum M2 based on a new screening strategy. An extracellular chitosanase was isolated and purified from the fermentation broth of Penicillium oxalicum M2. A 19.34-fold purification was achieved on a cation exchange column. Using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, chitosanase was determined at approximately 42 kDa without any subunits. The sequence of peptide in the protein was identified as SALNKNYITNFSTLR by MALTI-TOF/TOF MS. The maximum catalytic activity of the purified enzyme was 60.45 U/mg at the optimum pH and temperature of 5.5 and 60 degrees C. The enzyme activity held stability in the range of 35-50 degrees C and pH 3-4.5. Ca2+, Mn2+, non-ionic surfactants (Tween 20/40/60/80 and Trition X-100) and some common reducing agents (DTT and beta-ME) could significantly activate chitosanase. The purified enzyme showed rigorous specificity to chitosan as a substrate. The hydrolysate in the final stage of hydrolysis consisted of chitooligosaccharides with a degree of polymerization ranging from 2 to 5 and without glucosamine or acetylglucosamine. The monomeric enzyme obtained by one-step purification reveal applications potential in sugar industry, and expanded our understanding of the GH75 family chitosanases simultaneously.
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