A Method for High-Resolution Three-Dimensional Reconstruction with Ewald Sphere Curvature Correction from Transmission Electron Images

Article Optics

Adorym: a multi-platform generic X-ray image reconstruction framework based on automatic differentiation

Ming Du et al.

Summary: Adorym is an optimization-based X-ray image reconstruction framework that provides a generic forward model and supports parallel processing, allowing improved image quality through parameter refinement for various imaging methods.

OPTICS EXPRESS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Structure of the SARS-CoV-2 RNA-dependent RNA polymerase in the presence of favipiravir-RTP

Katerina Naydenova et al.

Summary: The structure of the RNA polymerase inhibitor favipiravir bound to the SARS-CoV-2 RNA-dependent RNA polymerase has been determined, revealing its binding mode at the catalytic site. The inhibitor is weakly incorporated into the RNA primer strand, leading to suppression of RNA replication in the presence of natural nucleotides.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

Ming Sun et al.

Summary: In high-resolution and high-throughput cryo-EM structure determination, detector technology plays a crucial role. The latest K3 camera offers improved throughput and resolution compared to its predecessor, with the correlated-double sampling mode (CDS) showing superior performance. Optimal dose rates and magnifications were determined, showcasing the efficiency of imaging protein complexes using the K3 direct detector.

JOURNAL OF STRUCTURAL BIOLOGY (2021)

添加到收藏夹

Article Microscopy

Relative roles of multiple scattering and Fresnel diffraction in the imaging of small molecules using electrons, Part II: Differential Holographic Tomography

T. E. Gureyev et al.

Summary: The paper presents a new electron microscopy reconstruction method called Differential Holographic Tomography, which can achieve three-dimensional atomic-level reconstruction for sparse weakly scattering structures, such as small biological molecules, using the incoherent first Born approximation. The method is capable of accurately reconstructing the locations of atoms in a molecule from TEM data and obtaining high-quality results with a small number of random orientations of the molecule.

ULTRAMICROSCOPY (2021)

添加到收藏夹

Article Microscopy

A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

J. P. J. Chen et al.

Summary: The algorithm developed is capable of reconstructing a three-dimensional object from a collection of two-dimensional images significantly influenced by the curvature of the Ewald sphere, particularly useful in cryoelectron microscopy when larger samples, higher resolution, or lower energy electron beams are desired.

ULTRAMICROSCOPY (2021)

添加到收藏夹

Article Microscopy