Biocompatible acid-degradable micro-mesoporous CaCO3:Si:Fe nanoparticles potential for drug delivery

Calcium carbonate nanoparticles of 50 nm in diameter are synthesized within the mesoporous silica particles with a subsequent etching out of the template material. Due to interaction of the filler with a template the as prepared CaCO3 nanoparticles are doped with Si which allows preserving their small size and porous structure. To make an aggregatively stable aqueous suspension of the nanoparticles they are additionally doped with Fe. The obtained CaCO3:Si:Fe nanoparticles possess a micro-mesoporous structure with specific surface area of 205 m(2) g(-1) and pore volume of 0.59 cm(3) g(-1). It is shown that the particles are fully dissolved in dilute HCl in several minutes. It is demonstrated that nanoparticles are nontoxic and are able to penetrate across the cell membrane and accumulate in HeLa and K-562 cells. Owing to high adsorption capacity a loading of 25 wt% doxorubicin (DOX) into CaCO3:Si:Fe nanopaticles is performed. Being internalized by cells DOX-loaded CaCO3: Si:Fe nanoparticles provide greater amount (up to three times) of DOX within the intracellular space compared to free doxorubicin. Encapsulation of the drug into the nanoparticles reduces its toxicity, IC50 value increases by 2.5 and 5 times for HeLa and K-562 cells, respectively.

Automated summary

Calcium carbonate nanoparticles with small size and porous structure were synthesized using a template method, and were additionally doped with Si and Fe for stabilizing aqueous suspension and enhancing cellular uptake. The nanoparticles were shown to dissolve completely in dilute HCl and possess high adsorption capacity for loading the anti-cancer drug doxorubicin. Encapsulation of the drug in the nanoparticles reduced its toxicity and increased its intracellular accumulation.